Journal: Scientific Reports

Article Title: Intracellular detection and communication of a wireless chip in cell

doi: 10.1038/s41598-021-85268-5

Figure Lengend Snippet: Cell containing intracellular RFID aligned within transceiver antenna loops for RFID detection and identification. The transceiver antennas are connected to a transmission line and probe pads used for electrical measurements. The insert shows an optical image of the RFID in a cell taken with a Keyence VK-X Series 3D Laser Scanning Confocal Microscope.

Article Snippet: The insert shows an optical image of the RFID in a cell taken with a Keyence VK-X Series 3D Laser Scanning Confocal Microscope.

Techniques: Transmission Assay, Microscopy

Journal: Biology

Article Title: ATP Purinergic Receptor P2X1-Dependent Suicidal NETosis Induced by Cryptosporidium parvum under Physioxia Conditions

doi: 10.3390/biology11030442

Figure Lengend Snippet: Live-cell 3D-holotomographic microscopy of PMN co-incubated with C. parvum (sporozoites/oocysts) in three dimensions. ( A ) is a time-lapse image of PMN stained with DRAQ5 (red) and SYTOX Green (green). ( B ) illustrates the 3D rendering, 3D holotomography picture, and 3D digital staining, of control experiment (inactivated PMN). Determination of digital staining using the refractive index (RI) values of the cell structures that were examined. The segmented nuclei region is shown in red. ( C ) Three-dimensional digital staining demonstrates the morphological alterations that parasite-exposed PMN undergo after 20, 80, and 150 min, correspondingly.

Article Snippet: Additionally, we used live cell three-dimensional holotomographic microscopy (3D Cell Explorer ® , Nanolive, Lausanne, Switzerland) to see and better comprehend the early contacts between bovine PMN and C. parvum oocysts/sporozoites throughout the dynamic NETotic process.

Techniques: Microscopy, Incubation, Staining, Control, Refractive Index